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Antibodies against ubiquitin (Cat# 3936, 1:400), AMPK (Cat# 2532 S, 1:1000), and p-AMPK (Cat# 2531 S, 1:1000) were purchased from Cell Signaling Technology

This "paradoxical response" (where treatment temporarily makes symptoms worse) includes: Numbness and tingling Shooting pains Joint pain Increased sensitivity Temporary inflammation (swelling and irritation) While these symptoms generally indicate that treatment is working (as nerves begin to repair themselves), they can be distressing for patients and may require additional treatment for comfort

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This protein must be present to combine with B12 in order for our bodies to absorb and use it

For example, the Vegetarian Nutrition Dietetic Practice Group recommends that vegans consider supplementing with 250 mcg of B12 per day

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