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Animal model metabolomics studies reveal that HFD-induced NAFLD is characterized by hepatic saturated fatty acid (myristic acid, palmitic acid) and monounsaturated fatty acid (oleic acid) accumulation, accompanied by decreased gluconeogenic amino acid (glycine, alanine, aspartic acid, glutamic acid, proline) levels (95)

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4 Discussion The severe insulin resistance, hepatic steatosis, dyslipidaemia and hyperphagia that characterises lipodystrophy makes effective treatment challenging and limits the effectiveness of commonly used diabetes therapies (13, 40, 41)

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