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Sixty male, 5-wk old, wild-type C57BL/6J mice (JanVier Labs, France) were group housed in a controlled environment (12 h lightdark cycle, 21 2 C, 50 10% humidity) and fed the AMLN diet high in trans-fat, cholesterol, and fructose 46 ad libitum for 32 weeks to induce obesity and the NASH phenotype

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Muscle protein is in a constant state of turnover, meaning that protein synthesis is occurring continuously to replace protein lost as a consequence of protein breakdown