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Then, iodoacetamide (IAA, Sigma-Aldrich, #I1149) or FA and/or L-buthionine-sulfoximine (L-BSO, Sigma-Aldrich, #B2515) and/or antioxidants were added to a final volume of 200 L per well

Research by Reinstatler and colleagues (2012, Diabetes Care) found that among people taking metformin at doses of 1,000 mg per day or more, the prevalence of B12 deficiency was 5.8 percent