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Methods Insect cell expression HA-signal peptide-FLAG-3C-GLP-1R-3C-8HIS 13 , human DNG s 46 , His 6 -tagged human G 1 and G 2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described

The calculated activation energies (G in kcal mol -1 ) for the abstraction the C2 proton of 7 to produce the cyanidin enolate ( 8-enolate) were found to be almost identical for the wild-type and the C13S variant, in agreement with their similar dehydratase activity measured experimentally

It was suggested that certain combinations, particularly a GHK-Cu to LMW HA ratio of 1:9, significantly increased the synthesis of collagen 4 compared to when either compound was examined alone

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