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Acknowledgements: This work was supported in part by NIA grant R01 AG057381 (Ladiges, PI)

Susceptibility to the following 10 antibiotics was measured: 5 mcg of ciprofloxacin, and rifampicin

The next day, samples were incubated with FITC-conjugated, TRITC-conjugated, or Cy5-conjugated (1:1000, Invitrogen, United States) secondary antibody along with 4,6-diamidino-2-phenylindole (DAPI) (1:1000, Sigma-Aldrich, United States) at room temperature for 1 h