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One of the significant downsides for me was the bloating and discomfort that came with the prebiotic fiber

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Proteins were transferred onto nitrocellulose membranes, which were then blocked by incubation with 5% milk (Rapilait) for 1 h at room temperature

To confirm its biological function, firstly we tried to measure the enzymatic activity of GpoA by comparison of total protein extracts obtained from wt and gpoA strains, but the reactivity of H 2 O 2 impeded to perform this enzymatic assay

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