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Akiyoshi et al [7] and Chappell et al [8] used layers of 10% and 25% and much milder centrifugation conditions of 4000 g for 30 min

doi: 10.1073/PNAS.0806720106 30 FerreiraJPSaraivaFSharmaAVasques-NvoaFAnglico-GonalvesALeiteARet al