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Let the vial rest for one to two minutes, then swirl gently again

Brtsch SH, Wang CC, Li L, Stender H, Neziroglu N, Richter C, et al

J Neurosci Res 91(8):10761083 Mangia S, Tkc I, Gruetter R, Van de Moortele PF, Maraviglia B, Uurbil K (2007) Sustained neuronal activation raises oxidative metabolism to a new steady-state level: evidence from 1 H NMR spectroscopy in the human visual cortex

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Observations were made in the morning, afternoon and evening of each day during the culture period to record the food intake, activity and mortality of the bullfrogs