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(3) and (3) respectively transforming the recombinant expression vectors obtained in the step (2) into escherichia coli BL21(DE3) through a heat shock method, screening monoclonals through resistance, respectively selecting 8 clones from the two recombinant engineering bacteria, respectively naming the clones as A1-GLP-1-1 to A1-GLP-1-8 and A2-GLP-1-1 to A2-GLP-1-8, and storing the clones in glycerol

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Journal of Biomedical Informatics 2019;95:103208