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The following secondary antibodies were used: Alexa Fluor 555-labelled donkey anti-rabbit IgG (H+L) (#A0453, Beyotime, Shanghai, China, 1:200), horseradish peroxidase (HRP)-labelled goat anti-rabbit IgG (H+L) (#GB23303-1, Servicebio, 1:8000), HRP-labelled goat anti-mouse IgG (H+L) (#GB23301, Servicebio, 1:8000), and HRP-conjugated goat anti-rabbit IgG (#D110,058, Sangon Biotech, 1:8000)

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PMCID: PMC5808442

What to avoid Certain combinations should be avoided or approached with extreme caution

The term describes the hollow, sunken, or aged appearance that some patients develop after significant weight loss, particularly when that loss happens quickly

Therefore, guided by the precautionary principle and concern for patient safety, advanced stages of chronic kidney disease should be considered a relative contraindication to the ketogenic diet until more studies become available