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The concentration of Human MPIF2 in the samples is then determined by comparing the OD of the samples to the standard curve

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An alignment of MrGST1 protein with anthocyanin-related GSTs identified in other dicotyledonous plants indicated that all of these GSTs had the conversed GST-N-Phi (Thioredoxin-like superfamily), GST-C-Phi (GSTC-family superfamily) domains, and high-homology sites (indicated as red boxed) in the GST family (Figure 1B)

In total, 500 g lysates were added to blocking buffer (HEN buffer with 2.5% SDS and 20 mM MMTS) at 50 C for 20 min with frequent vortex