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Microglia and astrocyte dysfunction in Parkinsons disease

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The sample was centrifuged at 13,000 g, 4 C for 10 min and the supernatant was desalted using concentrator PES, 3 K MWCO (Thermo Scientific) at 13,000 g , 4 C for 20 min

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doi: 10.1038/s41401-018-0158-8

N-acetyl cysteine or vitamin E) treatment to quench ROS, (3) co-immuno-fluorescence to detect colocalization of BHZ and LOXs in the samples