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KG), SDS was then added and the cells were mixed with the color reagent (TBA in acetic acid and sodium hydroxide) in a boiling water bath for 1 h, cooled in an ice-water bath, and then incubated on ice

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In vitro, intracellular GSH depletion made cancer cells more sensitive to oxidative stress, overcoming drug resistance and further improving outcomes establishing the GSH system as a key research target in oncology

Thiol-containing compounds (RSH) react with DTNB to form mixed disulfides (RS-S-DTNB), where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB

Some people experience mild digestive upset when first starting, especially on an empty stomach